Mumps IgG capture EIA: Cat. No: MuVG012
For the detection of mumps specific IgG antibodies in human oral fluid
In the Microimmune Mumps IgG capture EIA recombinant mumps nucleoprotein
(rMuNP) antigen is added to anti-human IgG coated microtitre wells. This
is followed by the addition of undiluted oral fluid extracted from a collection
device(e.g. "Oracol" or "OraSure") or diluted
serum / plasma. Mumps specific IgG in the specimen if present binds to
the anti-human IgG coated microwell.the well and to the rMuNP. After washing
the wells, a monoclonal antibody to the rMuNP, conjugated to horseradish
peroxidase is added. After washing, tetramethylbenzadine (TMB) substrate
is added. The presence of mumps specific IgG in the sample results in
a colour change in the TMB from colourless to blue. The enzymatic reaction
is terminated with acid which causes a further change in colour from blue
to yellow. The colour change and intensity is monitored using a spectrophotometric
plate reader set at 450nm with a correction filter between 620 and 650nm.
The presence of mumps specific IgM is indicated by optical density values
above the cut-off.
Features of the test:
- Assay time:2h
- Specimen type: oral fluid, serum / plasma
- Reagents: Colour coded and mostly ready to use.
- Shelf life: 12 months at 2..8°C
- *Sensitivity of the Microimmune Mumps IgM capture EIA on serum samples
compared to two competitor tests was 100% (95% CI 95.7% to100.0%) for
test 1and 94.3% (95% CI 88.1% to 97.9%) for test 2.
- *Specificity of MI test on serum samples compared to competitior test 1: was 94.2% (95% CI 84.1.13 to 98.9).
*A detailed description of the evaluation of the test is given in the product insert.
Status: To be launched September 2004.
||Mumps IgG Capture EIA
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